Photo-controllable encapsulation of protein using unnatural amino acid

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870291
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry; Bio-related chemistry
• Research Institution: Gifu University
• Principal Investigator: Ohno Satoshi 岐阜大学, 工学部, 助教 (10345796)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: タンパク質 / 非標準アミノ酸 / ナノカプセル

Outline of Final Research Achievements

Aquifex aeolicus lumazine synthase (AaLS) forms hollow-icosahedral capsule by self-assembling of 60 subunits. A negatively charged lumazine synthase mutant( AaLS_Neg ) is expected as a bio-nanocapsule which can be used for a DDS. In this study, we prepared several AaLS_Neg mutants, each carrying an azido-tyrosine at predetermined positions 22, 40, 44, 57, 91, respectively. The synthesized AaLS_Neg mutant containing azido-tyrosine at position 22 was covalently crosslinked via azide group by UV irradiation. And then, this photo-crosslinked product was separated on a gel filtration column. As a result, we observed that the encapsulation efficiency of photo-crosslinked product was increased.

Free Research Field

タンパク質工学