Identification of retrotransposons involved with genomic instability in tumors

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870298
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology; Tumor biology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: KAHYO Tomoaki 浜松医科大学, 医学部, 助教 (40416665)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: がん / ゲノム / 遺伝性疾患 / 内在性レトロウイルス / 高速DNAシーケンス / レトロトランスポゾン / 変異

Outline of Final Research Achievements

None has linked the retrotransposition of human endogenous retrovirus elements (HERVs) and genomic instability in tumors due to the technical difficulty to analyze repetitive sequences. In this study, we have developed the technology using the inverse PCR and high throughput sequencing methods, in which the flanking regions of HERVs were amplified and sequenced. In the analysis targeting HML-2, a group of HERV, approximately 50% of HML-2_LTR sites were reproducibly detected. Furthermore, multiple candidates of novel HML-2_LTR sites were also identified in tumor genomes. This result will help to reveal presence and functions of HML-2 retrotranspositions tumor genomes.

Free Research Field

がんゲノム