Imaging of Tissue Regeneration and Tissue Engineering using Bioavailable molecular probes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870359
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: Kimura Yu 京都大学, 健康長寿社会の総合医療開発ユニット, 特定准教授 (90566027)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分子プローブ / デンドリマー / 磁気共鳴イメージング / MRI / ガドリニウム

Outline of Final Research Achievements

Novel Gadolinium (Gd)-Magnetic Resonance Imaging (MRI) probes with high-sensitivity were succeeded to synthesize and introduced with functional groups for conjugation of target ligands to specific cells. Complex molecules of novel Gd-MRI contrast agent and antibody fragment or polyethyleneglycol (PEG) was then obtained after the reaction of reduced antibody fragment and activated-PEG. The affinity of cell-specific labeling was evaluated with quartz crystal microbalance measurement and the no change of antigen-binding ability was confirmed before and after the conjugation of Gd-MRI contrast agent. Through evaluation of binding affinity to cultured cells and of body distribution after in vivo administration, many findings to clarify the cell distribution in tissue regeneration process were obtained.

Free Research Field

バイオイメージング・高分子化学