NMR analysis of the conformationl change of an Arf-family Small G Protein

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry; Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Okamura Hideyasu 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50620928)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ダイナミクス / 新規拡張model-free解析法 / 無細胞蛋白質合成システム

Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of the conformational change of an Arf-family Small G protein by using NMR relaxation analysis. Nicotiana tabacum and Human Arl8s were produced by a cell-free system that is capable of Advanced isotope-labeling techniques. The relaxation data of Arl8s were obtained and analyzed. In addition, I developed an extended model-free analysis that is applicable to macromolecular high concentrated systems like biological cells.

Free Research Field

構造生物学