2014 Fiscal Year Final Research Report
Elucidation of DNA repair mechanisms of blue light receptor proteins
| Project/Area Number |
25870400
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | DNA修復 / DNA損傷 / 光生物 |
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| Outline of Final Research Achievements |
(6-4) photolyases are flavoproteins that are able to directly repair the (6-4) photoproducts, one of the UV-induced DNA lesions, in response to blue light in sunlight. Thus far, overall function of the (6-4) photolyase still remains obscure. Here, in order to investigate the DNA repair mechanism of the (6-4) photolyase, molecular scientific studies using mutants of the enzyme were performed. As results, several amino acids residues involved in the repair and recognition of the (6-4) photoproduct have been identified. In future, these information would be applied to the investigations on cryptochromes, which share high homologies with the (6-4) photolyase and are responsible for the regulation of the circadian clocks in human.
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| Free Research Field |
生物科学
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