2015 Fiscal Year Final Research Report
Gender differences of neutrophil extracellular traps and neutrophil apoptosis in septic patients.
| Project/Area Number |
25870422
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
Laboratory medicine
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | NETs / 性差 / 敗血症 / アポトーシス |
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| Outline of Final Research Achievements |
Neutrophils were isolated from septic patients and healthy volunteers and harvested for 4 hours. The apoptosis were assessed by Anexine V using flowcytometer, and the area of neutrophil extracellular traps (NETs) was assessed by immunofluorescence staining using anti-H1 antibody and anti-neutrophil elastase antibody. Neutrophils from healthy volunteers were stimulated by lipopolysaccharide. And the plasma cell-free DNA was assessed by realtime RT-PCR using human globin gene. Septic patients showed high cell-free DNA concentrations, low neutrophil apoptosis%, larger NETs areas. The apoptosis% of female was significantly lower than that of male in LPS-stimulated healthy volunteers’ neutrophils. NETs area of female tended to be higher than that of male in septic shock patients.
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| Free Research Field |
救急・災害医学
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