2015 Fiscal Year Final Research Report
Identification and functional analysis of novel vertebrate chromosomal proteins with using Multi-Classifier Combinational Proteomics
| Project/Area Number |
25870487
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
Molecular biology
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| Research Institution | Kochi University |
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Principal Investigator |
Ohta Shinya 高知大学, 教育研究部医療学系基礎医学部門, 特任助教 (00631194)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 染色体 / 分裂期 |
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| Outline of Final Research Achievements |
In this study we identified CENP-32. CENP-32 appears to be required for centrosomes to integrate into a fully functional spindle that notonly nucleates astral microtubules, but also is able to nucleate and bind to kinetochore andcentral spindle microtubules. Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-32 depletion, possibly due to an imbalance of forces within the spindle.
Next we identified CSAP and proposed that CSAP associates with MTs around centrosomes to stabilize MTs during mitosis, ensuring proper bipolar spindle formation and maintenance.
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| Free Research Field |
細胞生物学
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