Certification of LPS-DNA complex production by gram-negative bacteria and its high-toxicity in periodontitis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870505
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry; Periodontology
• Research Institution: Kyushu University
• Principal Investigator: MIURA Mayumi 九州大学, 大学病院, 研究員 (00404054)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: グラム陰性菌 / LPS-DNA複合体 / 歯周炎

Outline of Final Research Achievements

This study aimed to extract LPS-DNA complex from periodontal bacteria, and to analyze the pathogenicity. We succeeded in extracting DNA-LPS complex by using neutral phenol. It also cleared that bonding mode of LPS-DNA complex was both ionic and non-ionic bond. In addition, it was suggested that structure of the LPS affected to form LPS-DNA comlplex. Furthermore, comparing with the high-purity LPS, inflammatory cytokine TNF-α production by LPS-DNA complex was lower value. Further analysis is necessary for toxic expression of LPS-DNA complex.

Free Research Field

歯周病学