2015 Fiscal Year Annual Research Report
A genomic approach to the identification of transmission blocking antigens of malaria parasites
| Project/Area Number |
25870525
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| Research Institution | Nagasaki University |
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Principal Investigator |
カレトン リチャード 長崎大学, 熱帯医学研究所, 准教授 (10503782)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | malaria / genomics / bioinformatics / LGS / WGS / Gametocyte / vaccine |
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| Outline of Annual Research Achievements |
We have achieved the following results:
i)Initiating strain-specific transmission-blocking immunity in Plasmodium yoelii. We have performed numerous experiments using natural immunity to block transmission in P. yoelii. We have shown that there is a sharp decrease in the infectivity of gametocytes throughout the course of an infection in mice, and, using IFN-Gamma and MyD88 KO mice, that this is independent of the innate immune response. We have used sera transfer to demonstrate that sera from mice with long-term infections supresses the ability of early-infection stage gametocytes to transmit, and that this suppression has a strain-specific component.
ii)We are currently investigating the feasibility of performing LGS experiments with P. falciparum. Using the strains 3D7 and HB3, we have cultured pure gametocytes, and purified crude protein, to produce immune sera in rabbits. We have acquired the uncloned recombinant progeny of a cross between these two strains.
iii)We have developed bioinformatics and statistic techniques capable of identifying statistically significant deviations in the relative frequency of SNP markers measured in whole genome resequencing analyses of recombinant progeny populations of malaria parasites following selection.
iv)We have shown that there is no link between virulence and transmission success in malaria parasites.
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[Journal Article] Little Polymorphism at the K13 Propeller Locus in Worldwide Plasmodium falciparum Populations Prior to the Introduction of Artemisinin Combination Therapies2016
Author(s)
Mita T, Culleton R, Takahashi N, Nakamura M, Tsukahara T, Hunja CW, Zayar ZW, Hitake WW, Marma AS, Dysoley L, Ndounga M, Dzodzomenyo M, Akhwale WS, Kobayashi J, Uemura H, Kaneko A, Hombhanje F, Ferreira MU, Björkman A, Endo H, Ohashi J
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Journal Title
Antimicrobial Agents and Chemotherapy
Volume: 印刷中
Pages: 印刷中
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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