Elucidation of mechanism of plant-microbe interaction mediated by aromatic amino acid L-DOPA

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870604
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; Applied microbiology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: KOYANAGI Takashi 石川県立大学, 生物資源環境学部, 准教授 (20535041)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Pseudomonas 属 / 芳香族アミノ酸脱炭酸酵素 / ドーパ脱炭酸酵素 / ビタミンB6酵素 / アレロケミカル / アレロパシー / 根圏細菌

Outline of Final Research Achievements

Aromatic amino acid L-DOPA acts as an allelochemical in rhizosphere to cause the growth inhibition of other organisms. In this study, we investigated the role of a rhizospheric bacterium Pseudomonas putida for plant-microbe interaction via control of the concentration of L-DOPA. P. putida harbors aromatic L-amino acid decarboxylase (AADC) which specifically catalyzes the reaction converting L-DOPA into dopamine, through this reaction the allelopathy of L-DOPA is reduced. DNA microarray analysis revealed that only the expression of the AADC structural gene was drastically induced in the presence of L-DOPA, indicating the specific role of this bacterium through responding to L-DOPA. In addition, we obtained the candidate ORFs with unknown functions possibly encoding the specific factors regulating the AADC induction. Based on these results, we could expand the grasp of AADC-mediated symbiosis between P. putida and plant.

Free Research Field

応用微生物学