2014 Fiscal Year Final Research Report
Development of GBS rapid detection method for pregnant woman
| Project/Area Number |
25870706
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
Obstetrics and gynecology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | GBS / 妊婦 / 迅速診断 / 抗体 / B群溶血性連鎖球菌 |
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| Outline of Final Research Achievements |
Group B Streptococcus (GBS) is a leading cause of serious neonatal infections. CDC recommends GBS screening for all pregnant women. Although GBS screening has been performed mainly by the culture-based method, it takes several days to obtain a reliable result. In this study, we developed an immunochromatographic test (ICT) for the detection of GBS-specific surface immunogenic protein in 15 min from an overnight enrichment culture. This ICT was able to detect recombinant Sip levels 0.5 ng/ml, or about 10＾6 CFU/ml of GBS cells. The cross reactivity test using 26 species of microorganism showed no detectable false positive result. The accuracy of the ICT for GBS detection from enrichment cultures of vaginal swabs was compared with culture-based method. From the results using 260 specimens, the sensitivity and specificity of the ICT appeared 93.1% and 99.6%, respectively. The newly developed ICT is readily applicable to clinical use in the detection of GBS.
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| Free Research Field |
感染症学
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