Enhancement of mechanical properties of stem cell-based self-assembled tissue as a novel medical material for ligament and tendon

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870723
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; Orthopaedic surgery
• Research Institution: Seikei University (2015); Tokai University (2013-2014)
• Principal Investigator: OYA KEI 成蹊大学, 理工学部, 助教 (50549962)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 幹細胞自己生成組織 / 間葉系幹細胞 / 腱・靭帯 / ナノ周期構造 / フェムト秒レーザ加工 / 動的ひずみ負荷培養 / 静的荷重負荷培養

Outline of Final Research Achievements

A stem cell-based self-assembled tissue (scSAT) biosynthesized using synovium-derived mesenchymal stem cells is expected as a novel medical material for ligament and tendon. However, it is necessary to improve the mechanical properties of the scSAT for clinical application. In this study, the scSAT was synthesized on a nanoperiodic structured substrate patterned via a femtosecond laser processing. In addition, dynamic strain and static loading were loaded on the MSCs and their extracellular matrix (ECM) during scSAT synthesis. It was demonstrated that all techniques improved the mechanical properties of the scSAT. The tissue orientation of the scSAT was controlled by the special culturing on a nanoperiodic substrate and loading of dynamic strain. On the other hand, the thickness of the scSAT was thickened by static loading. From these results, it is considered that tissue orientation and enhancement of ECM synthesis contribute to the improvement of mechanical properties of the scSAT.

Free Research Field

再生医工学