Analysis of the role of RNA surveillance on protein qality control in the endoplasmic retisulum

2016 Fiscal Year Final Research Report

• Project/Area Number: 25870761
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology; Functional biochemistry
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Sakaki Kenjiro 東京女子医科大学, 医学部, 講師 (70509968)
• Research Collaborator: Mitani Shohei 東京女子医科大学, 医学部, 教授 (90192757) ; Moizumi Sawako (Yoshina Sawako) 東京女子医科大学, 医学部, 講師 (00424672)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 細胞内品質管理機構 / 小胞体ストレス応答

Outline of Final Research Achievements

Intracellular quality control system surveys gene products to ensure the accuracy of gene expression. Furthermore, interaction of quality control systems promotes cell adaptation in various stress conditions. Because intracellular quality control is tightly associated with many diseases including diabetes, neuronal degeneration and tumorigenesis, the molecular mechanism of quality control systems is expected to be elucidated.
We recently published the novel interaction of RNA surveillance system and protein quality control in the endoplasmic reticulum (ER) that is known as ER quality control. The defects in nonsense-mediated mRNA decay (NMD) provokes UPR activation in the both of mammalian cells and the nematode C.elegans.
In here, we analyzed the role of NMD-associated genes by using C.elegans, and revealed that the expand of UPR activation by NMD deficiency depends on mutant alleles. This observation might be important to elucidate its role and mechanism in UPR activation.

Free Research Field

細胞生物学