2014 Fiscal Year Final Research Report
Mechanisms of epigenetic regulation via lncRNA transcription at the imprinted Zdbf2 locus
| Project/Area Number |
25870766
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
Genome biology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Genomic imprinting / DNA methylation / Epigenetics / lncRNA / Embryogenesis / Placenta |
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| Outline of Final Research Achievements |
Parent-of-origin DNA methylation pattrens at imprinted genes are established during gametogenesis and maintained after fertilization. In this study, we focus on epigenetic regulation system of Zdbf2 imprinted gene. Here, we identified meternally methylated Gpr1 primary (germline-derived) DMR and Zdbf2 secontdary (somatic) DMR at the imprinted Zdbf2 region. We also demonstrated that the epigenetic regulation mechanisms via lncRNA, i. e., lncRNA or GPR1AS, were well-conserved between human and mouse genomes. Our findings provide a new model system for dynamic shaping DNA methylation patterns in mammalian genomic imprinting.
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| Free Research Field |
エピゲノム
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