2014 Fiscal Year Final Research Report
Roll of acidic organelle membrane fusion factor (Vam8) involved in trophoblast stem cell growth signal
| Project/Area Number |
25870902
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
Embryonic/Neonatal medicine
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| Research Institution | Himeji Dokkyo University (2014)
Doshisha Women's College of Liberal Arts (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 栄養膜幹細胞 / 酸性オルガネラ / シグナル伝達 |
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| Outline of Final Research Achievements |
In mammalian cells, acidic organelles play roles in transportation and degradation of the signaling molecule that is involved in cell growth. In the process, complex consisting of multiple membrane fusion factors regulates membrane fusion between organelles. We have previously obtained the Vam8 gene deficient mice that reveal specific lack of placental tissue (trophectoderm) in embryos.
In this research, we tried to establish trophoblast stem cell (TS cell) lines from the conditional Vam8 gene deficient mice. Then, we examined the influence of Vam8 gene deficiency on the expression and activities of the signaling molecule that regulate TS cell growth. As a result, we found that Vam8 regulate TS cell growth by mTOR-independent mechanism.
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| Free Research Field |
細胞生物学
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