2016 Fiscal Year Final Research Report
Proteomic analysis of chemotherapy resistance related proteins in human breast cancer cells
| Project/Area Number |
25870924
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
Applied pharmacology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Tanaka Satoru 大阪医科大学, 医学部, 非常勤講師 (50595741)
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| Research Collaborator |
Sakai Akiko 大阪医科大学, 医学部, 講師
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | プロテオーム / 乳癌 / タキサン |
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| Outline of Final Research Achievements |
To identify the proteins involved in paclitaxel resistance, we compared the proteomes of MCF-7 human breast cancer cells and its paclitaxel-resistant subclone MCF-7/PTX. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry, many proteins were modulated between these cells. Among them, PROTEIN A were upregulated in MCF-7/PTX cells. Knockdown of PROTEIN A using small interfering RNA in MCF-7/PTX cells restored their sensitivity to paclitaxel.
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| Free Research Field |
腫瘍学
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