2015 Fiscal Year Final Research Report
A comprehensive analysis of small RNAs involved in environmental adaptation and pathogenicity of Vibrio parahaemolyticus
| Project/Area Number |
25870987
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
Bacteriology (including mycology)
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| Research Institution | Matsuyama University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 腸炎ビブリオ / 小分子RNA / 転写後制御 / 鉄獲得系 / Ⅲ型分泌装置 |
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| Outline of Final Research Achievements |
It is known in bacteria that expression of genes involved in environmental adaptation is regulated at the level of transcription as well as post-transcriptionally. In this study, I examined the post-transcriptional control of the genes necessary for adaptation to iron starvation in Vibrio parahaemolyticus. As a result, it was revealed that the V. parahaemolyticus peuA gene encoding a receptor for siderophore enterobactin, which is a high affinity iron chelator engaged in iron acquisition, is regulated by translational control. Moreover, I studied whether the genes encoding type III secretion system (T3SS), which is a major virulence factor of V. parahaemolyticus, are regulated post-transcriptionally. As a result, it was disclosed that the small RNA Spot 42 post-transcriptionally regulates the expression of VP1682 in V. parahaemolyticus, which functions as a chaperone for the T3SS effector VP1680.
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| Free Research Field |
微生物学、衛生薬学、分子生物学
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