2015 Fiscal Year Final Research Report
Development and Analytical Applications of Pretreatment Technique utilizing Fluorous and Non-Covalent Interactions
| Project/Area Number |
25870994
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
Physical pharmacy
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | フルオラスケミストリー / 溶媒抽出 / ヌクレオチド / 細胞試料 |
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| Outline of Final Research Achievements |
A novel and selective extraction method for nucleotides based on non-covalent fluorous biphasic extraction system in the presence of perfluoroalkylamine as an ion-pair reagent. The ion-pairs between nucleotides and perfluoroalkylamine were selectively extracted to a fluorous solvent via the affinity of perfluoroalkyl-containing compounds with each other, i.e. fluorous interaction. Then, the nucleotides in the fluorous solvent could be back-extracted with aqueous ammonia in acetonitrile as non-fluorous solvent, and the extracts were directly injected to LC system. A hydrophilic interaction chromatography was utilized for separation of the nucleotides by gradient elution with the mixture of acetonitrile, water, and ammonium carbonate buffer as mobile phase. This method was successfully applied to the determination of nucleotides in some cell samples.
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| Free Research Field |
分析化学
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