Identification of direct target molecules of bioactive natural compounds using monoclonal antibodies against natural compounds

2015 Fiscal Year Final Research Report

• Project/Area Number: 25871011
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines; General internal medicine(including psychosomatic medicine)
• Research Institution: Nagasaki International University
• Principal Investigator: UTO Takuhiro 長崎国際大学, 薬学部, 講師 (90469396)
• Research Collaborator: SHOYAMA Yukihiro 長崎国際大学, 薬学部, 教授 (70037604) ; MORINAGA Osamu 第一薬科大学, 薬学部, 准教授 (60465771) ; FUJII Shunsuke 長崎国際大学, 健康管理学部, 助教 (10610165)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生薬成分 / 標的分子 / モノクローナル抗体 / オウゴン / バイカレイン / 甘草 / リクイリチン

Outline of Final Research Achievements

The aim of this study was to elucidate the series of behaviors of natural compounds in herbal medicines, such as accumulation, cellular localization, and binding to target molecules, using monoclonal antibodies against bioactive natural compounds. 1) Baicalein exerts anti-proliferation and anti-inflammatory activities by binding to a protein of approximately 70kDa in organelles. 2) Liquiritin and liquiritigenin activate melanin synthesis by binding to a 35-38kDa protein. These approaches may make it possible to determine the detailed mechanism of the action of bioactive natural compounds in herbal medicines.

Free Research Field

生薬学、天然物化学