Optimization of fusion gene identification methods

2015 Fiscal Year Final Research Report

• Project/Area Number: 25871073
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics; Respiratory organ internal medicine
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: Togashi Yuki 公益財団法人がん研究会, 分子標的病理プロジェクト, 研究助手 (00648016)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: バイオマーカー / 融合遺伝子

Outline of Final Research Achievements

A) We compared lung tumors harboring fusion genes, EGFR or KRAS mutation, and those without the aberrations by global mRNA expression assay. Several highly-expressed genes were identified in ALK and ROS1 fusion-positive lung cancers. B) We developed an RNA capture sequencing system to detect the fusion genes and SNV/indels frequently observed in lung cancers. The system’s performance was excellent on both fresh frozen and formalin-fixed, paraffin-embedded specimens.

Free Research Field

分子生物学