Development of cesium-accumulating mutant of radioresistant bacteria by ion beam breeding technology

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Modeling and technologies for environmental conservation and remediation; Quantum beam science
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: SATOH Katsuya 独立行政法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究副主幹 (90370402)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 微生物バイオレメディエーション技術 / 放射線抵抗性細菌 / イオンビーム突然変異育種技術 / 放射性セシウム / デイノコッカス・ラジオデュランス / 環境修復 / 環境保全 / 突然変異株

Outline of Final Research Achievements

We conducted the development of high Cs-accumulating mutant from Deinococcus radiodurans using ion beam breeding technology. D. radiodurans were mutagenized with ion beams that were the highest mutant frequency observed (1E-1 to 1E-2 of surviving-fraction). The imaging analysis using Cs-137 was performed to estimate the Cs-accumulating ability of the mutagenized cells. Candidates of Cs-accumulating mutant were screened on the basis of the luminescent intensity and the cell area as indicators of intracellular Cs level and cell growth, respectively. As a result, we obtained 33 candidates, whose Cs-accumulating abilities were over 2-fold higher than that of the wild-type, from the population of mutagenized cells (about 3,000). Although more detailed analysis regarding the Cs-accumulating ability and the sensitivity to gamma rays are needed, these candidates generated by ion beam breeding technology could be expected to use for bioremediation purpose.

Free Research Field

放射線微生物学