Analyses of transcriptional network regulating secondary wall biosynthesis in tall fescue

2015 Fiscal Year Final Research Report

• Project/Area Number: 25871101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding; Crop production science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Sato Hiroko 国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター作物開発研究領域, 主任研究員 (50391363)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トールフェスク / 牧草 / 細胞壁 / 消化性 / 遺伝子

Outline of Final Research Achievements

Forage digestibility decreases with secondary wall deposition during stem development. To analyze transcriptional network regulating secondary wall biosynthesis in forage grasses, suppression subtractive hybridization was used to identify differentially expressed genes between wild-type tall fescue and transgenic one expressing OsSWN2 chimeric repressor that caused a reduction of cell wall thickening in sclerenchyma cells. After differential screening, differentially expressed genes were categorized into various functional categories according to their putative functions by BLAST analysis. Two transcription factors, FaMYB and FaNAC, were isolated as candidate genes involved in secondary wall biosynthesis. To characterize their function, transgenic tall fescue was generated by using CRES-T.

Free Research Field

遺伝育種学