2014 Fiscal Year Final Research Report
Establishment of novel pluripotent stem cells in mammals by iPS technology.
| Project/Area Number |
25871115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
General medical chemistry
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| Research Institution | Shiga University of Medical Science (2014)
Institute of Physical and Chemical Research (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | iPS細胞 / 異種間キメラ / 新型多能性幹細胞 / INTPS細胞 / ラットiPS細胞 / TS細胞 / トランスポゾン / piggyBac |
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| Outline of Final Research Achievements |
We developed a comprehensive system for generation and evaluation of iPS cells and generated functional rat germ cells from rat iPS cells in mouse-rat interspecific chimeras.
Additionally, we generated a novel pluripotent stem cells named INTPS cells by culturing ES cells in EpiSC medium containing a GSK3 inhibitor. The INTPS cells have intermediate feature between ES cells and EpiSCs and have an ability to contribute to chimeras and their germline.
We proposed that this culture condition is useful for generating novel pluripotent stem cells in non-rodent species, because this culture condition enables us to establish pluripotent stem cells independently from LIF signaling.
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| Free Research Field |
幹細胞
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