Development of real-time detection method of mRNA dynamics for the study of signal regulation system during left-right asymmetry formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871128
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology); Developmental biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TAKAI Akira 独立行政法人理化学研究所, 生命システム研究センター, 特別研究員 (60637205)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: シグナル伝達 / 発光イメージング / RNAイメージング

Outline of Final Research Achievements

In this research, we developed brighter, cyan and orange variants of Nano-lantern, a recently developed super-brilliant yellowish-green luminescent protein which is suitable for in vivo imaging. These multicolor Nano-lanterns enable monitoring of multiple biological events, including dynamics of intracellular microstructures, gene expressions, and calcium ion concentrations. Furthermore, we developed real-time mRNA detection method by combining Nano-lantern with RNA binding proteins. After further improvement of this mRNA detection method, we will apply it in a study of signal regulation mechanisms during left-right asymmetry formation.

Free Research Field

発生生物学、細胞生物学