Development of a data processing system for LC-QqQ/MS based metabolomics

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Life / Health / Medical informatics; Biomolecular chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TSUGAWA Hiroshi 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (30647235)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: メタボロミクス / データ処理 / 化合物同定 / 質量分析

Outline of Final Research Achievements

We developed a data processing pipeline for widely targeted metabolomics by using the multiple reaction monitoring (MRM) mode. Widely targeted metabolomics became increasingly popular in metabolomics for the simultaneous analysis of several hundred metabolites at high sensitivity and selectivity. However, the identification criterion to distinguish the target metabolites from the isomeric metabolites or background noise has not been developed. In addition, a user-friendly software to deal with the large-scale data sets is required. MRMPROBS was therefore developed for the assessment of large-scale MRM data applicable to any instrument or experimental conditions.

Free Research Field

メタボロミクス