2014 Fiscal Year Final Research Report
Development of the method for controlling stem cell fate
| Project/Area Number |
25871140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
Developmental biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 光遺伝学 / mRNA / 翻訳 / 細胞分化 / 再生医療 |
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| Outline of Final Research Achievements |
The use of light as an external stimulus offers the potential for spatiotemporal control and is thus ideal for controlling protein expression in living cells. We have developed a reversible method for regulating protein expression using a photoresponsive-cap that can control the translation of mRNA in a reversible manner by triggering the cis-trans photoisomerization of the cap. We succeeded in controlling the amount, timing and duration of protein expression in living mammalian cells. Additionally, neuronal differentiation of PC12 cells was photo-induced by controlling constitutively active H-Ras 61L protein expression.
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| Free Research Field |
ケミカルバイオロジー
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