Studies on Structural and Positional Identification of Isomeric Constituents in Crude Drugs using Ion Mobility Spectrometry

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines; Drug development chemistry
• Research Institution: National Institute of Health Sciences
• Principal Investigator: MASADA Sayaka 国立医薬品食品衛生研究所, 生薬部, 主任研究官 (50647097)
• Research Collaborator: TERASAKA Kazuyoshi 名古屋市立大学, 大学院薬学研究科・生薬学分野, 講師 (60405214)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 構造異性体 / 質量分析 / 生薬 / 配糖体

Outline of Final Research Achievements

We focused on the isomeric glucosides in crude drugs to establish a new strategy for the identification of isomeric constituents using liquid chromatography ion mobility spectrometry mass spectrometry (LC-IMS-MS). We performed LC/IMS/MS analysis of crude drugs and detected some isometric glucosides with drift times obtained from IMS. Then, actual collision cross sections (CCS) calculated by drift times were compared to theoretical CCS simulated by molecular models to presumptively identify the peaks. Anthraquinone glucosides, iridoid glucosides and tetracyclic alkaloids were successfully matched the presumative identification with referencial compounds with little difference of actual vs theoretical CCS.

Free Research Field

生薬学