Roles of spinal dorsal horn TRP channels in neuropathic pain

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871214
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology; Pain science
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: ZHOU Yiming 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特別協力研究員 (20647888)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 生理学

Outline of Final Research Achievements

S-palmitoylation is a reversible post-translational modification that can control the surface expression, spatial organization, interactions, and functional activities of proteins. However, little is known about whether and how TRP channels are regulated by S-palmitoylation. We investigated the role of S-palmitoylation in the in vitro and in vivo activity of several TRP channels. Treatment with a palmitoyl acyltransferase (PAT) enzyme inhibitor 2-bromopalmitate (2BP) altered the agonist-induced responses of TRPM8, TRPA1, and TRPV2 channels in HEK293T cells. Meanwhile, co-expression of TRP channels with the global PAT enzyme DHHC7, but not DHHC3, significantly increased the responses of TRPM8 and TRPA1. Moreover, treatment with 2BP and N-tert-butyl-hydroxylamine (NtBHA), a chemically cleaving reagent of thioester linkage, decreased the responses and percentages in a population of endogenous TRPM8 and TRPA1. Thus, expression of some TRP channels are regulated by S-palmitoylation.

Free Research Field

分子細胞生理学