Preparation of vascularized thick myocardial tissue by using in-body cell self-organization inducible technology

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; General surgery
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: IWAI Ryosuke 独立行政法人国立循環器病研究センター, 研究所, 特任研究員 (60611481)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 再生医療 / 組織工学 / 自己組織化

Outline of Final Research Achievements

Although the tissue-engineered myocardial tissues prepared by fabricating cardiomyocytes are considered to be a promising transplant for cardiac regeneration, large part of these tissues was not able to function as expected because the central ischemic necrosis was occurred in them. In this study, we developed in-body cell self-organization inducible technology to prepare vascularized myocardial tissues. Tubular tissues having 0.5 mm thickness containing cardiomyocytes layer and abundant nutrient capillary vessels were obtained by only subcutaneous placement of tubular acrylic mold embedded with cardiomyocytes. On the other hand, we successfully prepared cells aggregates containing gene-transfected cells by only seeding cells on gene-deposited culture surfaces using our developed CAT polymer. The combination of in-body cell self-organization inducible technology with gene-transfected cells aggregates might enable us to develop native-like high-density myocardial tissue.

Free Research Field

細胞工学