Analysis in molecular mechanism of transcriptional activation of LRH-1 by beta-catenin

2014 Fiscal Year Final Research Report

• Project/Area Number: 25882050
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biomolecular chemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: YUMOTO Fumiaki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 特任准教授 (30360150)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: LRH-1 / X線結晶構造解析 / X線小角散乱解析

Outline of Final Research Achievements

LRH-1 plays an essential role in early development as it is expressed in embryonic stem cells. It is a transcription factor expressed in liver, pancreas, and ovary to regulate gene expressions. In addition to the biological aspect, it has been recognized as a drug target because the receptor is involved in multiple cancer developments. Full-length LRH-1 and N-terminal region-truncated LRH-1 were produced in E. coli and complexes of the protein-DNA duplex were further purified by gel filtration after elution from Ni-NTA column. The complex formation with DNA was critical to handle the sample for structural study and for biochemical characterization. LRH-1-DNA-peptide ternary complex was also prepared. The samples have been tested in small angle X-ray scattering and crystallization with the samples. The results suggested that the both LRH-1 molecules such as full-length and N-terminal truncated version had “bar” type shape.

Free Research Field

構造生物学