Research on the development of fluorescent three-dimensional motion-picture holographic microscopy

2014 Fiscal Year Final Research Report

• Project/Area Number: 25886014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Optical engineering, Photon science
• Research Institution: Kansai University
• Principal Investigator: TAHARA Tatsuki 関西大学, システム理工学部, 助教 (50709095)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: ディジタルホログラフィ / ホログラフィ / ディジタルホログラフィック顕微鏡 / 複数波長ディジタルホログラフィ / 蛍光イメージング / 多重記録 / 位相分割多重記録技術 / 蛍光ディジタルホログラフィ

Outline of Final Research Achievements

In this research, (i) the development of an incoherent three-dimensional holographic recording technique and the proposal of a system with high light efficiency, (ii) signal processing techniques for reconstructing a high-quality three-dimensional image, (iii) simultaneous recording of multiple wavelengths with high light efficiency using the technique of (i), and (iv) experimental evaluation of the resolution in digital holographic microscopy were conducted. In (i), the technique was numerically simulated and its validity was confirmed. Also, a novel type of the incoherent system was proposed. In (ii) and (iii), it was clarified that signal processing and multiwavelength imaging techniques can be applied (i). In (iv), it was experimentally demonstrated that digital holographic microscopy has the ability for imaging 775 nm apertures with a single-shot exposure although the experimental evaluation of an incoherent holographic microscope was not experimentally conducted yet.

Free Research Field

光工学・光量子科学