2014 Fiscal Year Final Research Report
Dynamic conversion of MSC differentiation by photo-regulating surface properties of cell adhered substrate
| Project/Area Number |
25889060
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Composite materials/Surface and interface engineering
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | 光架橋性 / 高分子ブラシ表面 / 分化 / 間葉系幹細胞 |
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| Outline of Final Research Achievements |
In this study, we designed a photo cross-linkable polymer brush surface having dimethylmaleimide moieties (DMI) for dynamically regulating cell behaviours of hbmMSCs. By UV irradiation, mobility of polymeric chain and nanotopology of these surfaces are able to change with photodimerization reaction of DMI. Photo cross-linkable polymer brush surfaces of poly((dimethyl maleimide ethyl methacrylate)-co-(methyl methacrylate)) [sPDMIM] were prepared by surface-initiated RAFT polymerization. To evaluate the effect of photo-induced alteration of the surface properties on cell behaviours of hbmMSCs, the cells were cultured on the sPDMIM surfaces with or without UV irradiation. hbmMSCs took spindle shape without UV cross-linking, although the cells widely spread on cross-linked sPDMIM surfaces. Furthermore, qRT-PCR assays showed that hbmMSCs on sPDMIM surfaces expressed mRNA of osteocalcin 1.8 times higher than those on cross-linked sPDMIM surfaces at 7-day cultivation.
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| Free Research Field |
バイオマテリアル
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