Molecular mechanism of temporal AMPA-type glutamate receptor internalization during hippocampal long-term potentiation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25890009
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Hiromitsu 京都大学, 理学(系)研究科(研究院), 助教 (30705447)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 神経科学 / 分子生物学 / ライブイメージング / シナプス可塑性 / 長期増強 / 長期抑圧 / グルタミン酸受容体 / 全反射顕微鏡

Outline of Final Research Achievements

The efficacy of synaptic transmission changes depending on the pattern of neuronal activity. This synaptic plasticity has been regarded as a cellular basis of learning and memory. In this study, we have suggested that the amount of a subtype of AMPA-type glutamate receptors (AMPA receptors) on postsynaptic membrane is reduced together with dynamin during hippocampal long-term potentiation by experiments using total internal reflection fluorescence microscopy. In addition, we have developed a new method to visualize individual endocytosis of AMPA receptors.

Free Research Field

神経細胞生物学