Generation and characterization of monoclonal antibodies against the extracellular regions of taste receptors

2014 Fiscal Year Final Research Report

• Project/Area Number: 25891017
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Okayama University
• Principal Investigator: YASUI Norihisa 岡山大学, 医歯(薬)学総合研究科, 助教 (90467514)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 抗体 / 味覚受容体 / Fab断片 / GPCR / 相互作用

Outline of Final Research Achievements

The sense of tastes by human and other animals depends on the recognition of tastant molecules by taste receptor proteins. In this project, we generated and characterized monoclonal antibodies against the extracellular regions of taste receptors. We have established the method to prepare the high-quality Fab fragments from IgGs and analyzed their interaction with recombinant proteins of the extracellular regions of taste receptors by using biochemical/biophysical techniques. We confirmed that the Fab fragments prepared here had the ability to bind the extracellular regions of taste receptors with high affinity.

Free Research Field

タンパク質化学，タンパク質工学