2014 Fiscal Year Final Research Report
New insight into the function of the guard cell chloroplast by a genetic approach
Project/Area Number |
25891020
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Plant molecular biology/Plant physiology
|
Research Institution | Kyushu University |
Principal Investigator |
NEGI JUNTARO 九州大学, 理学(系)研究科(研究院), 特任助教 (70529099)
|
Project Period (FY) |
2013-08-30 – 2015-03-31
|
Keywords | 気孔 / 葉緑体 / CO2 / 環境応答 / シロイヌナズナ |
Outline of Final Research Achievements |
Guard cell chloroplasts have been proposed to play an important role in the osmoregulatory mechanisms mediating stomatal movements, although their function has been a subject of debate and remains to be confirmed. In this study, I isolated an Arabidopsis gles1 mutant that had non-chlorophyllous stomata. CO2 and light dependent stomatal responses were severely disturbed in the non-chlorophyllous stomata of the gles1 mutant. Whole-cell patch-clamp experiments indicated that in the mutant guard cells, CO2 activation of the S-type anion channels was impaired. These results provide direct evidence to support the idea that guard cell chloroplasts are essential for light-induced stomatal opening and CO2-induced stomatal closure.
|
Free Research Field |
植物分子生理学
|