2013 Fiscal Year Annual Research Report
The molecular mechanism and cellular function of interaction between oil bodies and peroxisomes
| Project/Area Number |
25891029
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
CUI Songkui 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (20712532)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | organelle interaction / lipid degradation / oil body / peroxisome / sucrose |
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| Research Abstract |
The microscopic observation of oil body-peroxisome interaction revealed that the oil bodies and peroxisomes make strong interaction during the seedlings break down the storage lipid, showing the correlation between the organelle interaction and lipid degradation. By analyzing the sucrose defective mutant sdp1, it was shown in our study that the sucrose, which is the final product of lipid degradation, might act as a signal to reduce the oil body-peroxisome interaction. Consistently, in vitro interaction assay showed the enhanced interaction of these two organelles in this mutant. This system can be used in the future to qualify the oil body-peroxisome interaction.
The mutants which represent the potential candidates as anchor proteins were prepared. The peroxisomes and oil bodies were visualized by crossing these mutants with OleG/ThiR plants to identify the anchor protein from the candidates.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Characterisation of peroxisome and oil bodies are accomplished in wild type and sdp1. The plants which are used for identification of candidate as anchor proteins are prepared. Election microscopic analysis is under progress.
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| Strategy for Future Research Activity |
To test whether sucrose act as a specific signal molecule or as the general metabolites, the different kinds of sugars with different concentrations will be tested for their effects on the oil body-peroxisome interaction.
The plan will be mainly aimed at the identification and characterization of an anchor protein that tethers peroxisomes to oil bodies.
The membrane structure at the contact site between two organelles will be observed more clearly using electron microscope. Furthermore, the immuno-electron microscopic analysis will be performed using the antibody against anchor protein candidate in order to understand the subcellular location and interacting site on these two organelles.
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