2014 Fiscal Year Final Research Report
Identification of short and branched-chain aldehyde reductases and its application for unnatural biochemical production
| Project/Area Number |
25892021
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | 1,3-ブタンジオール / ブタノール / 3-ヒドロキシブチルアルデヒド / 大腸菌 / 合成代謝経路 / アルコール脱水素酵素 / 代謝工学 / 合成生物工学 |
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| Outline of Final Research Achievements |
The fermentation conditions including pH and overall oxygen transfer coefficient (kLa) were optimized to improve 1,3-butanediol production by metabolically engineered Escherichia coli. AdhP, EutG, and YqhD in E. coli were identified as alcohol dehydrogenases that catalyze the reduction of 3-hydroxybutyraldehyde to 1,3-butanediol. A CoA-dependent butanol synthetic pathway that functions under aerobic conditions was constructed in E. coli, by expanding the previously reported 1,3-butanediol synthetic pathway.
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| Free Research Field |
微生物代謝
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