2013 Fiscal Year Annual Research Report
Effect of polyfluorinated compounds exposure and PPAR alpha pathway polymorphisms on maternal BMI and lipid profile, birth weight, growth and development of infants in Japan
| Project/Area Number |
25893004
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| Research Institution | Hokkaido University |
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Principal Investigator |
GOUDARZI Houman 北海道大学, 環境健康科学研究教育センター, 学術研究員 (40713607)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | Prefluorinated chemicals / PPAR / Birth outcome / Child growth |
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| Research Abstract |
This study is a part of "The Hokkaido Study on Environment and Children's Health" as a prospective birth cohort. In this study, our objective is to evaluate the epidemiological effect of maternal PFCs exposure and peroxisome proliferator activating-receptors pathway polymorphisms on maternal lipid profile, birth outcome (birth weight, birth size), and children’s growth until school age.Prenatal levels of PFC compounds including PFOA and PFOS are available in maternal serum samples (n=514) between 2002 and 2005. Concentration of these chemicals were measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). Primers for SNPs determination of following gens were purchased: PPARs (α, β, and γ), Leptin, LXR, HNF4α, and CAR. 400 μl of maternal whole blood samples which are collected immediately after delivery were subjected to the genomic DNA extraction. Using EP1 system (Fluidigm), I am examining SNP genotyping by end-point reading after PCR method. This process will be finished until 2014, September. After finishing genetic analysis, we assess the effect of prenatal PFCs exposure in underlying genetic background (SNPs in PPAR and PPAR-related pathways) on maternal TG and fatty acids during pregnancy. It may result in low birth size, and birth weight. Also, we are going to observe longitudinal effects of these chemical and genetic factors on child’s growth until school age. Therefore, we can identify of high risk groups classified by genetic susceptibility to PFCs exposure as a novel national pattern of environment-genetic interaction in Japanese population.
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| Current Status of Research Progress |
Reason
翌年度、交付申請を辞退するため、記入しない。
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| Strategy for Future Research Activity |
翌年度、交付申請を辞退するため、記入しない。
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Research Products
(2 results)
