The measurement of cell death in human neural stem cells and glioblastoma cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893030
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: ISONO Mayu 群馬大学, 重粒子線医学推進機構, 研究員 (90713511)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 神経幹細胞 / 脳腫瘍細胞 / ネクローシス / アポトーシス / 放射線

Outline of Final Research Achievements

We analyzed the level of IR-induced cell death in neural stem and glioblastoma cells. To measure the type of cell death, i.e. necrosis and apoptosis, after IR, we carried out Acridine Orange and Ethidim Bromide assay. We observed that neural stem cells show greater IR sensitivity than glioblastoma cells. Imporantly, neural stem cells preferentially undergo apoptosis after IR. Further, we observed greater p53 phosphorylation in neural stem cells compared with that in glioblastoma cells. In addition, we performed a preliminary study on the effect of bystander in these cells. We found less effect of bystander in terms of cell death in neural stem cells.

Free Research Field

放射線生物学