Functional analysis of SPAG4, a novel target of hypoxia-inducible factor (HIF) in the kidney

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893045
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: SHOJI Kumi 東京大学, 医学部附属病院, 助教 (00439423)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 低酸素 / 低酸素誘導因子 / 尿細管

Outline of Final Research Achievements

I assessed the expression pattern and the function of sperm-associated antigen 4 (SPAG4) which is a novel target of hypoxia-inducible factor, in the kidney. First, I revealed that hypoxia upregulates the expression of SPAG4 in the renal tubular cells both in vivo and in vitro. Next I generated SPAG4 conventional knockout mice using Cre/Lox P system for the first time. After ischemia-reperfusion injury, there were no significant differences in renal function and in renal histology between SPAG4 knockout mice and littermate control mice. The function of SPAG4 in the kidney remains to be solved.

Free Research Field

腎臓内科学