Functional analysis of human LMIR3/CD300F

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893050
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Collagenous pathology/Allergology
• Research Institution: The University of Tokyo
• Principal Investigator: IZAWA Kumi 東京大学, 医科学研究所, 助教 (80708313)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: マスト細胞 / アレルギー / IgE

Outline of Final Research Achievements

Leukocyte mono-immunoglobulin-like receptor (LMIR) belongs to a paired activating and inhibitory receptor family. Mouse LMIR3 (mLMIR3)/CD300f
is an inhibitory receptor containing 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a single immunoreceptor tyrosine-based switch motif (ITSM).We have recently demonstrated that extracellular ceramide is a ligand for mLMIR3/CD300f, which is a negative regulator of high-affinity IgE receptor (FcεRI)-mediated activation of mast cells. Although ceramide is a major ligand for mLMIR3, both sphingomyelin and ceramide act as physiological ligands for hLMIR3 in mast cells, thereby inhibiting FcεRI-mediated activation of mast cells. These results will help understand the intrinsic mechanism by which excessive activation of mast cells is prevented in human beings, leading to the development of novel therapeutic strategies against allergic diseases.

Free Research Field

医歯薬学