2014 Fiscal Year Final Research Report
Analysis of intracellular localization of periodontal pathogen in gingival epithelial cells
| Project/Area Number |
25893120
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | 歯学 |
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| Outline of Final Research Achievements |
<Objective> In this study, we focused on the molecular basis underlying intracellular localization of Porphyromonas gingivalis (Pg), a pathogenic bacterium of periodontitis, in gingival epithelial cells. It has been reported that vesicle-associated membrane protein (VAMP2), one of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor, has a key role in the fusion of transport vesicles to their target membrane. The objective of study is to determine the involvement of VAMP2 in intracellular localization of Pg. <Results> At 1 hour post-infection (hpi), about half of intracellular Pg was co-localized with VAMP2 in early endosomes in human immortalized gingival epithelial cells (HIGECs). Knockdown of VAMP2 increased accumulation of Pg in HIGECs, and caused accumulation of Pg in early endosomes even up to 5 hpi. <Conclusion> VAMP2 is suggested to be involved in the intracellular trafficking of Pg from early endosomes to distinct organelles in gingival epithelial cells.
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| Free Research Field |
医歯薬学
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