Analgesic mechanism of Botulinum toxin in neuropathic pain

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893140
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Okayama University
• Principal Investigator: MARUHAMA Kotaro 岡山大学, 医歯(薬)学総合研究科, 助教 (60712792)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: ボツリヌス毒素 / 神経伝達物質 / 三叉神経 / 神経障害性疼痛 / 軸索輸送

Outline of Final Research Achievements

BoNT/A heavy chain (Hc) was labeled with Thiol-Reactive Probes (Alexa-488) and injected into the dissociated rat trigeminal ganglion somata and the rat facial skin. Localization of BoNT/A-Hc was monitored in trigeminal ganglion neurons by confocal laser scanning microscopy.
BoNT/A-Hc was taken up by trigeminal ganglion neurons. This uptake was inhibited by colchicine treatment, which blocks axonal transport. Our data suggest that retrograde axonal transport of BoNT/A to sensory ganglion occurs following peripheral administration.

Free Research Field

医歯薬学