2014 Fiscal Year Final Research Report
Analysis of ameloblastoma tumor microenvironment and development of novel therapies
| Project/Area Number |
25893141
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
TAKEBE Yuichiro 岡山大学, 医歯(薬)学総合研究科, 助教 (00714677)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
|
| Keywords | エナメル上皮腫 / CCN2 |
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| Outline of Final Research Achievements |
35 paraffin-embedded ameloblastoma cases, ameloblastoma-derived cell lines (AM-1) and primary cultures of ameloblastoma stromal fibroblasts were used. Immunohistochemistry, Western blot and RT-PCR were performed. Parenchyma-stromal CCN2 overexpression correlated significantly with fibrous-type compared to myxoid-type stroma, suggesting a role in fibrosis .Recombinant CCN2 induction of enhanced ASF proliferation in AM-1 medium supports this view.Results suggest that effects of secreted growth factors are governed by ameloblastoma parenchyma-stromal interactions. CCN2 promotes fibrogenesis independent of TGF-β signaling. Its ablation is associated with a phenotypic switch to a myxoid-type microenvironment, conducive for TGF-β/BMP4 signaling to promote osteoclastogenesis.
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| Free Research Field |
口腔病理学
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