Identification of master regulator on TNBC malignant using TAT protein system

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893153
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Yamaguchi University
• Principal Investigator: WATANABE Kenji 山口大学, 大学研究推進機構, 助教 (50711264)
• Research Collaborator: MIZUKAMI Yoichi ; URUSHIMA Yutaka
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: TNBC / TAT protein

Outline of Final Research Achievements

Triple-negative breast cancer (TNBC) is one of breast cancer subtype and often shows more malignant phenotype compared to other subtype of breast cancer. Although there are many reports about TNBC, the master regulator involved in malignancy of TNBC is unclear. In this study, we paid our attentions to TNBC and tried to identify the master regulator involved in proliferation activity of TNBC. To achieve our purpose, we extracted the proteins from TNBC cell line, MDA-MB-231 cells, and fractionated them using ion-exchange chromatography. We transfected the each fractionated proteins into HCC38 cell by TAT system, and measured cell proliferation activity. However, the remarkable change was not detected.

Free Research Field

分子生物学