2014 Fiscal Year Final Research Report
Analysis of SNPs in artificially degraded DNA
| Project/Area Number |
25893256
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
MITSUYO Machida 東京女子医科大学, 医学部, 特任助教 (60468692)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | SNP解析 / 劣化DNA |
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| Outline of Final Research Achievements |
DNA in biological fluids at the scene is often degraded by many environmental factors. Short tandem repeats profiling, the most commonly used method for forensic DNA identification, is sometimes difficult to analyze degraded DNA samples. Here we focused on single nucleotide polymorphisms (SNPs) because the amplicon sizes are as short as those of degraded DNA in SNP analysis. To identify the SNPs that are resistant to degradation, we screened the SNPs from reported SNP lists and isolated the SNPs by amplified fragment length polymorphism analysis. Therefore six SNPs (rs891700, rs2111980, rs1360288, rs2116020, rs1636218, rs12293045) were demonstrated as detectable SNPs for degraded DNA samples.
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| Free Research Field |
法医学
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