The differentiation ability in the undifferentiated stem cell from the human dental pulp cells of deciduous teeth

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893273
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KAWAI Saki 大阪歯科大学, 歯学部, 助教 (70707067)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 歯髄 / 乳歯 / 未分化

Outline of Final Research Achievements

In the past study, it was reported that 6-bromoindirubin-3’-oxime（BIO）maintains undifferentiation ability in mouse and human ES cell. I examined the cell proliferation and undifferentiation ability in human dental pulp cells of deciduous teeth (hDPCs) was cultured with cell culture medium and BIO. We used MTS assay, Real time RT-PCR and Frow cytometory. We sorted hDPCs stimulated with BIO by Flow cytometry, but there was not the difference between hDPCs stimulated with BIO and hDPCs. Therefore I should examine the different culture condition. I investigate the undifferentiation ability of hDPCs in Hypoxia culture.

Free Research Field

医歯薬学