Molecular analysis of neuropathogenesis of mumps virus by reverse genetics system

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893295
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KATOH Hiroshi 国立感染症研究所, その他部局等, 研究員 (80711712)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: ムンプスウイルス / Pタンパク質 / ユビキチン-プロテアソーム / Heat shock protein 70

Outline of Final Research Achievements

In order to clarify the mechanisms of mumps virus (MuV) pathogenesis, we searched for host factors associated with the P protein, which is a viral protein essential for virus propagation, and investigated their roles in MuV infection. Heat shock protein 70 (Hsp70) was identified as a binding partner of the P protein. Hsp70 was recruited to the site of viral RNA replication, and its expression was increased during MuV infection. Although knockdown of Hsp70 using siRNAs had little effect on viral propagation, Hsp70 was suggested to regulate degradation of the P protein through the ubiquitin-proteasome pathway.

Free Research Field

ウイルス学