2016 Fiscal Year Final Research Report
Studies on genome integrity of archaea in the extremophilic environments
| Project/Area Number |
26242075
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
内海 利男 新潟大学, 自然科学系, 教授 (50143764)
金井 昭夫 慶應義塾大学, 環境情報学部, 教授 (60260329)
跡見 晴幸 京都大学, 工学(系)研究科(研究院), 教授 (90243047)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 超好熱性アーキア / ゲノム安定性維持 / 遺伝情報 / DNA修復 / RNAプロセシング / 翻訳装置 / 極限環境 / Thermococcales |
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| Outline of Final Research Achievements |
We discovered novel repair enzymes from hyperthermophilic archaea and named them EndoQ and EndoMS. EndoQ works for deaminated base repair. EndoMS is expected to be a repair enzyme for mismatched bases. These findings are the breakthrough for understanding of DNA repair systems in Archaea. By constructing an in vitro reconstitution system using PfEndA, PfRtcB, PfArchaease, and precursor tRNA, understanding of the reaction mechanism of tRNA processing has greatly progressed. PF0374 and PF0560 were discovered as novel regulation factors in the translation process. Their ribosome-binding property and each specific function were elucidated. With these discoveries, understanding of the mechanisms for genome integrity under extreme environments has greatly advanced.
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| Free Research Field |
生物分子化学
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