Fundamental research for genome-wide platform of industrial mammalian cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 26249125
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University (2015-2017); The University of Tokushima (2014)
• Principal Investigator: Omasa Takeshi 大阪大学, 工学研究科, 教授 (00252586)
• Co-Investigator(Kenkyū-buntansha): 西島 謙一 名古屋大学, 工学研究科, 准教授 (10262891) ; 河原 正浩 東京大学, 大学院工学系研究科(工学部), 准教授 (50345097)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: CHO cell / mammalian cell / antibody production / genome-wide analysis / chromosome

Outline of Final Research Achievements

The fundamental research of chromosome stability and selection of targeting region of genome, and analysis of signaling pathway for Chinese hamster ovary cells are necessary for genome-wide platform of industrial mammalian cells. We focused on the chromosome stability and find that BRCA1 repression is effective for the construction of stable and productive cell lines. Moreover, we investigated genome-wide gene expression among the CHO cell lines which have various chromosome numbers and determined the differentially-expressed genes among these cell lines and estimated the functions. Also, we determined the suitable target chromosomal region for site-specific genome integration.

Free Research Field

生物化学工学、動物細胞工学